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c6 36  (ATCC)


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    ATCC c6 36
    C6 36, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c6 36/product/ATCC
    Average 99 stars, based on 1391 article reviews
    c6 36 - by Bioz Stars, 2026-03
    99/100 stars

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    Adaptation and characterization of D2-vFLM D2-FLM. A) Immunofluorescent staining of DV2 and D2-FLM infected Vero-81 cells with a-NS3 antibody at 24 hours post-infection. B) Combined staining of prM and E of DV-2 and D2-FLM infected Vero-81 cells at 32°C and 37°C at 5 days post-infection. C) Schematic of D2-vFLM mammalian cell adaptation. Multiple-step growth curves of DV2, D2-FLM, and D2-vFLM in D) Vero-81 and <t>E)</t> <t>C6/36</t> cells. F) Western blot of DV2 and D2-vFLM with a-E and a-prM antibodies. Right: over-exposure to visualize prM in D2-vFLM. G) Quantification of prM:E ratio of D2-vFLM, normalized to DV2. Growth curves were analyzed by multiple t-test comparison between DV2 and D2-vFLM at different time points. Western blot quantification was analyzed by Students t- test.
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    Adaptation and characterization of D2-vFLM D2-FLM. A) Immunofluorescent staining of DV2 and D2-FLM infected Vero-81 cells with a-NS3 antibody at 24 hours post-infection. B) Combined staining of prM and E of DV-2 and D2-FLM infected Vero-81 cells at 32°C and 37°C at 5 days post-infection. C) Schematic of D2-vFLM mammalian cell adaptation. Multiple-step growth curves of DV2, D2-FLM, and D2-vFLM in D) Vero-81 and <t>E)</t> <t>C6/36</t> cells. F) Western blot of DV2 and D2-vFLM with a-E and a-prM antibodies. Right: over-exposure to visualize prM in D2-vFLM. G) Quantification of prM:E ratio of D2-vFLM, normalized to DV2. Growth curves were analyzed by multiple t-test comparison between DV2 and D2-vFLM at different time points. Western blot quantification was analyzed by Students t- test.
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    Adaptation and characterization of D2-vFLM D2-FLM. A) Immunofluorescent staining of DV2 and D2-FLM infected Vero-81 cells with a-NS3 antibody at 24 hours post-infection. B) Combined staining of prM and E of DV-2 and D2-FLM infected Vero-81 cells at 32°C and 37°C at 5 days post-infection. C) Schematic of D2-vFLM mammalian cell adaptation. Multiple-step growth curves of DV2, D2-FLM, and D2-vFLM in D) Vero-81 and <t>E)</t> <t>C6/36</t> cells. F) Western blot of DV2 and D2-vFLM with a-E and a-prM antibodies. Right: over-exposure to visualize prM in D2-vFLM. G) Quantification of prM:E ratio of D2-vFLM, normalized to DV2. Growth curves were analyzed by multiple t-test comparison between DV2 and D2-vFLM at different time points. Western blot quantification was analyzed by Students t- test.
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    Adaptation and characterization of D2-vFLM D2-FLM. A) Immunofluorescent staining of DV2 and D2-FLM infected Vero-81 cells with a-NS3 antibody at 24 hours post-infection. B) Combined staining of prM and E of DV-2 and D2-FLM infected Vero-81 cells at 32°C and 37°C at 5 days post-infection. C) Schematic of D2-vFLM mammalian cell adaptation. Multiple-step growth curves of DV2, D2-FLM, and D2-vFLM in D) Vero-81 and E) C6/36 cells. F) Western blot of DV2 and D2-vFLM with a-E and a-prM antibodies. Right: over-exposure to visualize prM in D2-vFLM. G) Quantification of prM:E ratio of D2-vFLM, normalized to DV2. Growth curves were analyzed by multiple t-test comparison between DV2 and D2-vFLM at different time points. Western blot quantification was analyzed by Students t- test.

    Journal: bioRxiv

    Article Title: Fusion Loop Modified and Mature Dengue Virus Elicits Protective Serum with Minimal Antibody Dependent Enhancement

    doi: 10.64898/2026.02.18.706631

    Figure Lengend Snippet: Adaptation and characterization of D2-vFLM D2-FLM. A) Immunofluorescent staining of DV2 and D2-FLM infected Vero-81 cells with a-NS3 antibody at 24 hours post-infection. B) Combined staining of prM and E of DV-2 and D2-FLM infected Vero-81 cells at 32°C and 37°C at 5 days post-infection. C) Schematic of D2-vFLM mammalian cell adaptation. Multiple-step growth curves of DV2, D2-FLM, and D2-vFLM in D) Vero-81 and E) C6/36 cells. F) Western blot of DV2 and D2-vFLM with a-E and a-prM antibodies. Right: over-exposure to visualize prM in D2-vFLM. G) Quantification of prM:E ratio of D2-vFLM, normalized to DV2. Growth curves were analyzed by multiple t-test comparison between DV2 and D2-vFLM at different time points. Western blot quantification was analyzed by Students t- test.

    Article Snippet: Aedes albopictus C6/36 cells (ATCC CRL-1660) were cultured in MEM (Gibco) supplemented with 5% FBS (HyClone), 1% penicillin/streptomycin (Gibco), 0.1 mM nonessential amino acids (Gibco), 1% HEPES (Gibco), and 2 mM GlutaMAX (Gibco) at 32°C with 5% CO 2 .

    Techniques: Staining, Infection, Western Blot, Comparison

    Compensatory mutations in structural proteins are important for Vero-81 adaptation. A) Virus yield of D2-vFLM-strucutral and -nonstructural infectious clones grown on C6/36 cells. Multiple-cycle growth curves of B) Vero grown or C) C6/36 grown virus D2-vFLM-strucutral and - nonstructural viruses on Vero-81 cells.

    Journal: bioRxiv

    Article Title: Fusion Loop Modified and Mature Dengue Virus Elicits Protective Serum with Minimal Antibody Dependent Enhancement

    doi: 10.64898/2026.02.18.706631

    Figure Lengend Snippet: Compensatory mutations in structural proteins are important for Vero-81 adaptation. A) Virus yield of D2-vFLM-strucutral and -nonstructural infectious clones grown on C6/36 cells. Multiple-cycle growth curves of B) Vero grown or C) C6/36 grown virus D2-vFLM-strucutral and - nonstructural viruses on Vero-81 cells.

    Article Snippet: Aedes albopictus C6/36 cells (ATCC CRL-1660) were cultured in MEM (Gibco) supplemented with 5% FBS (HyClone), 1% penicillin/streptomycin (Gibco), 0.1 mM nonessential amino acids (Gibco), 1% HEPES (Gibco), and 2 mM GlutaMAX (Gibco) at 32°C with 5% CO 2 .

    Techniques: Virus, Clone Assay